CD4+淋巴细胞亚群的表型变化与从抑制功能向辅助诱导功能的转变相关。
Phenotypic changes in a CD4+ lymphocyte subset correlate with a conversion from suppressor to helper inducer function.
作者信息
Nesbitt A M, Jones D B, Moore K
机构信息
University Department of Pathology, Southampton General Hospital, U.K.
出版信息
Immunology. 1990 Jan;69(1):65-70.
The monoclonal antibodies WR16 (CD45RA) and WR19 were used to isolate two reciprocal subsets of CD4 lymphocytes by negative selection. The CD4+/WR16-/WR19+ subset exhibited helper-inducer activity in a pokeweed mitogen (PWM)-driven Ig secretion assay, whereas the CD4+/WR16+/WR19- subset showed suppressor activity. When the CD4+/WR16+/WR19- subset was cultured in phytohaemagglutinin (PHA) or purified protein derivative (PPD) there was increased expression of the antigen binding WR19 but the cells remained WR16 negative. Cultures of CD4+/WR16+/WR19- lymphocytes with PHA or PPD induced a phenotypic change that resulted in a population which was CD4+/WR16-/WR19+. Investigation of the functional activity of the CD4+/WR16+/WR19- subset, before and after activation and phenotypic change to WR16-/WR19+, indicated that the change in phenotype correlated with a functional conversion from suppressor to helper-inducer activity. These results suggest that the CD4+/WR16+/WR19- suppressor subset comprises cells that differentiate to WR16-/WR19+ helper-inducer cells after exposure to antigen or mitogen.
单克隆抗体WR16（CD45RA）和WR19用于通过阴性选择分离CD4淋巴细胞的两个相互对应的亚群。在商陆有丝分裂原（PWM）驱动的Ig分泌试验中，CD4+/WR16-/WR19+亚群表现出辅助诱导活性，而CD4+/WR16+/WR19-亚群则显示出抑制活性。当CD4+/WR16+/WR19-亚群在植物血凝素（PHA）或纯化蛋白衍生物（PPD）中培养时，抗原结合性WR19的表达增加，但细胞仍为WR16阴性。用PHA或PPD培养CD4+/WR16+/WR19-淋巴细胞会诱导表型变化，导致产生一个CD4+/WR16-/WR19+的群体。对CD4+/WR16+/WR19-亚群在激活并向WR16-/WR19+表型变化之前和之后的功能活性进行研究，结果表明表型变化与从抑制活性向辅助诱导活性的功能转变相关。这些结果表明，CD4+/WR16+/WR19-抑制亚群包含在接触抗原或有丝分裂原后分化为WR16-/WR19+辅助诱导细胞的细胞。
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