丝裂霉素-C处理的反应细胞对单向混合淋巴细胞反应的抑制作用研究。
Studies on the suppression of a one-way mixed lymphocyte reaction by mitomycin-C treated responder cells.
作者信息
Victorino R M, Hodgson H J
出版信息
Immunology. 1980 Jul;40(3):447-53.
We have investigated the suppression of lymphocyte proliferation in a one-way mixed lymphocyte culture by autologous mitomycin-C treated responder cells. The suppression was proportional to the number of mitomycin-C treated cells added. Irradiated cells suppressed in the same manner. Using subpopulations of peripheral blood mononuclear cells, it was shown that the 'suppressor activity' was not confined to a particular subpopulation, but was exhibited by all subpopulations tested. Cycloheximide-treated peripheral blood mononuclear cells and autologous erythrocytes also suppressed. These results suggest that 'suppression' in this system is due to mechanical interference by the added cells. The implications for the design of assays for spontaneous suppressor cell activity in man are discussed.
我们研究了经丝裂霉素-C处理的自体反应细胞在单向混合淋巴细胞培养中对淋巴细胞增殖的抑制作用。这种抑制作用与添加的经丝裂霉素-C处理的细胞数量成正比。经照射的细胞也以同样的方式产生抑制作用。利用外周血单个核细胞亚群，结果表明“抑制活性”并不局限于某一特定亚群，而是在所测试的所有亚群中均有表现。用环己酰亚胺处理的外周血单个核细胞和自体红细胞也具有抑制作用。这些结果提示，该系统中的“抑制作用”是由于添加的细胞产生的机械干扰所致。文中还讨论了这些结果对人类自发性抑制细胞活性检测方法设计的意义。
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