Farrant J, Newton C
Clin Exp Immunol. 1981 Sep;45(3):504-13.
Concanavalin A (Con A) induced suppression of human peripheral lymphocytes has been studied in vitro at different cell concentrations using a 20 microliters inverted Terasaki plate culture system in which the uptake of 3H-thymidine was measured. Conventional assessment of suppression relates the response of cells to Con A in the presence of Con A-pretreated autologous cells, with the response of cells preincubated without Con A (control cells). The level of suppression so calculated was affected both by serum-related high background counts and by autologous stimulation of responding cells by mitomycin-treated Con A-pretreated cells. The high background was largely removed by the use of serum-free Iscove's medium. The data involving different cell concentrations and periods of cultivation indicate that the assessment of suppression is inadequate. Both Con A-pretreated and control cells are shown to 'help' the response of autologous responder cells to added soluble Con A. When the help by Con A-treated cells is less marked than that by control cells, apparent suppression is seen since the control cell provide the conventional baseline from which suppression is judged. After longer periods of cultivation the Con A-pretreated cells increased responses more than did the control cells leading to apparent enhancement.
在体外，使用20微升倒置的寺崎板培养系统，在不同细胞浓度下研究了伴刀豆球蛋白A（Con A）诱导的人外周淋巴细胞抑制作用，该系统中测量了³H-胸腺嘧啶核苷的摄取。传统的抑制评估方法是将在Con A预处理的自体细胞存在下细胞对Con A的反应与未用Con A预孵育的细胞（对照细胞）的反应进行比较。如此计算出的抑制水平受血清相关的高背景计数以及丝裂霉素处理的Con A预处理细胞对反应细胞的自体刺激的影响。通过使用无血清的伊斯科夫培养基，很大程度上消除了高背景。涉及不同细胞浓度和培养时间的数据表明，抑制评估是不充分的。Con A预处理细胞和对照细胞均显示出对添加的可溶性Con A的自体反应细胞的反应有“促进”作用。当Con A处理细胞的促进作用不如对照细胞明显时，就会出现明显的抑制，因为对照细胞提供了判断抑制作用的传统基线。培养较长时间后，Con A预处理细胞比对照细胞更能增强反应，从而导致明显的增强。
