粗糙脉孢菌NADP特异性谷氨酸脱氢酶的氨基酸序列。胃蛋白酶和胰凝乳蛋白酶肽段及完整序列。
The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence.
作者信息
Holder A A, Wootton J C, Baron A J, Chambers G K, Fincham J R
出版信息
Biochem J. 1975 Sep;149(3):757-73. doi: 10.1042/bj1490757.
Abstract
Peptic and chymotryptic peptides were isolated form the NADP-specific glutamate dehydrogenase of Neurospora crassa and substantially sequenced. Out of 452 residues in the polypeptide chain, 265 were recovered in the peptic and 427 in the chymotryptic peptides. Together with the tryptic peptides [Wootton, J. C., Taylor, J. G., Jackson, A. A., Chambers, G. K. & Fincham, J. R. S. (1975) Biochem. J. 149, 749-755], these establish the complete sequence of the chain, including the acid and amide assignments, except for seven places where overlaps are inadequate. These remaining alignments are deduced from information on the CNBr fragments obtained in another laboratory [Blumenthal, K. M., Moon, K. & Smith, E. L. (1975), J. Biol. Chem. 250, 3644-3654]. Further information has been deposited as Supplementary Publication SUP 50054 (17 pages) with the British Library (Lending Division), Boston Spa, Wetherby, W. Yorkshire LS23 7BQ, U.K., from whom copies may be obtained under the terms given in Biochem. J. (1975) 145, 5.
摘要
从粗糙脉孢菌的NADP特异性谷氨酸脱氢酶中分离出胃蛋白酶和胰凝乳蛋白酶肽段，并进行了大量测序。在多肽链的452个残基中，胃蛋白酶肽段回收了265个，胰凝乳蛋白酶肽段回收了427个。连同胰蛋白酶肽段[伍顿，J.C.，泰勒，J.G.，杰克逊，A.A.，钱伯斯，G.K.和芬奇姆，J.R.S.（1975年），《生物化学杂志》149卷，749 - 755页]，这些确定了该链的完整序列，包括酸性和酰胺基团的归属，但有七个位置重叠不足。其余的序列比对是根据另一个实验室获得的关于溴化氰片段的信息推导出来的[布卢门撒尔，K.M.，穆恩，K.和史密斯，E.L.（1975年），《生物化学杂志》250卷，3644 - 36
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