Tu S I, Nagahashi G, Sliwinski B J
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1367-73. doi: 10.1016/0006-291x(84)91242-7.
Plasma membrane enriched microsomal fraction was isolated from corn root cells by sucrose density centrifugation. The ATPase activity as measured by the release rate of inorganic phosphate, was decreased by the presence of modifiers which included diethylstilbestrol, vanadate, N,N'-dicyclohexylcarbodiimide, and miconazole. The presence of ADP also decreased the rate of ATP hydrolysis. Furthermore, a preincubation of the membrane with ADP significantly reduced the inhibitory effects of these membrane ATPase modifiers. Since the modes of interaction of these modifiers with the enzyme are different, the results suggest that the binding of ADP may stabilize the plasma membrane ATPase in a modifier insensitive state.
通过蔗糖密度离心法从玉米根细胞中分离出富含质膜的微粒体部分。以无机磷酸盐释放速率测定的ATP酶活性，因存在包括己烯雌酚、钒酸盐、N，N'-二环己基碳二亚胺和咪康唑在内的调节剂而降低。ADP的存在也降低了ATP水解的速率。此外，膜与ADP预孵育显著降低了这些膜ATP酶调节剂的抑制作用。由于这些调节剂与酶的相互作用方式不同，结果表明ADP的结合可能使质膜ATP酶稳定在对调节剂不敏感的状态。
