Carlson G M
Biochim Biophys Acta. 1984 Sep 25;789(3):347-50. doi: 10.1016/0167-4838(84)90191-2.
The number of molecules of an irreversible inhibitor that are responsible for inactivation of a catalytic site is often deduced from the slope of a plot of the log of the apparent rate of inactivation (k') at different concentrations of inhibitor versus the log of the inhibitor concentrations. The purpose of this note is to urge caution in experimental design and interpretation if one attempts to utilize this kinetic technique to characterize the order of inactivation brought about by functionally irreversible inhibitors that initially bind reversibly to an enzyme in the process of inactivation. Representative literature cases which have utilized plots of log k' versus log [I] for this type of inactivation are discussed.
导致催化位点失活的不可逆抑制剂分子数量，通常是根据在不同抑制剂浓度下，表观失活速率（k'）的对数与抑制剂浓度的对数作图的斜率推导得出。本注释的目的是提醒，如果有人试图利用这种动力学技术来表征功能上不可逆抑制剂引起的失活顺序，在实验设计和解释时要谨慎。这类失活过程中，抑制剂最初与酶可逆结合，本文讨论了利用log k'对log [I]作图的代表性文献案例。
