Mixed leukocyte reaction, graft-versus-host reaction, and skin allograft rejection in the lizard, Chalcides ocellatus.

In optimal seasonal conditions, splenocytes derived from adult lizards, Chalcides ocellatus, responded to vigorous proliferation in two- and one-way mixed leukocyte reaction cultures (MLRC). Data based on a large number of reciprocal MLRC provided evidence for the presence of strong and diverse lymphocyte activating determinants. These findings were fully confirmed in studies of systemic graft-versus-host reaction as intraperitoneal injection of splenocytes into newborn allogeneic recipients consistently induced splenomegaly, retarded growth and mortality. In favourable ambient conditions, adult lizards were also able to reject skin allografts in a subacute manner (mean survival time +/- S.E. = 28.8 +/- 0.88). The results clearly indicate that the lizard, Chalcides ocellatus, is endowed with an advanced type of cell-mediated immunity, and possesses strong and polymorphic histocompatibility antigens. Cellular alloreactivity in MLRC and towards skin grafts was, however, abrogated in winter and significantly diminished during spring through mid-summer as compared to mid-summer till autumn. The present study, thus, suggests that immunological defects attributed to reptiles might be more apparent than real, and essentially ascribable to the fact that the immune capacity of these ectothermic vertebrates is profoundly modulated by environmental conditions.