Khansari N, Fudenberg H H
Immunol Commun. 1984;13(4):351-60. doi: 10.3109/08820138409048669.
Human peripheral blood B-cells can be stimulated with PWM and antigen to produce specific antibody in vitro. This stimulation depends on the presence of T-cells and antigen. T cells, however, can be replaced by a soluble factor derived from a 48-hr culture of T-cells with either PWM and/or antigen. The helper factor, in the absence of antigen, acts as a polyclonal activator causing minimal proliferation of B-cells. When antigen is present, production of specific antibody is not dependent on the source of helper factor. Removal of monocytes abolished synthesis of both Ig and specific antibody although antigen and/or helper factor were present. While production of total IgG required autologous monocytes, the origin of the helper factor was not crucial. Production of specific antibody required that both monocytes and helper factor be derived from the same donor; therefore it seems that cooperation of B-, T-cells and monocytes for production of specific antibody is probably Ia restricted. In contrast, for production of polyclonal Ig (in the absence of antigen), cooperation of B-cells and monocytes with T-cells is not.
人外周血B细胞可用PWM和抗原在体外刺激产生特异性抗体。这种刺激依赖于T细胞和抗原的存在。然而，T细胞可用来自T细胞与PWM和/或抗原进行48小时培养的可溶性因子替代。在无抗原时，辅助因子作为多克隆激活剂，引起B细胞的最小增殖。当有抗原存在时，特异性抗体的产生不依赖于辅助因子的来源。去除单核细胞可消除Ig和特异性抗体的合成，尽管存在抗原和/或辅助因子。虽然总IgG的产生需要自体单核细胞，但辅助因子的来源并不关键。特异性抗体的产生要求单核细胞和辅助因子都来自同一供体；因此，B细胞、T细胞和单核细胞协同产生特异性抗体可能受Ia限制。相比之下，对于多克隆Ig的产生（无抗原时），B细胞、单核细胞与T细胞的协同不受此限制。
