Gerrard T L, Bonnard G D, Fauci A S
Cell Immunol. 1983 May;78(1):64-72. doi: 10.1016/0008-8749(83)90260-5.
The accessory cell requirements for the induction of proliferative and specific antibody responses of human lymphocytes stimulated with either antigen or mitogen were examined. An Ia-negative human myeloid tumor cell line, K562, could substitute for monocytes in the proliferation of monocyte-depleted lymphocytes in response to pokeweed mitogen (PWM) stimulation. K562 cells could also act as accessory cells in the PWM-induced anti-keyhole limpet hemocyanin (KLH) antibody synthesis of cells from a KLH-immunized donor. In contrast, only monocytes and not K562 cells could function as accessory cells in antigen-induced lymphocyte proliferation as well as in antigen-induced, antigen-specific antibody production. However, K562 cells, like monocytes, were able to positively and negatively regulate polyclonal immunoglobulin responses. Thus, Ia-bearing accessory cells can function in antigen-induced proliferation and antibody responses while non-Ia-bearing cells can function in mitogen-induced, but not antigen-induced responses. These studies indicate a dichotomy in the nature of required accessory cells in antigen-induced versus mitogen-induced human lymphocyte responses and strongly suggest an obligatory role of Ia or an Ia-related molecule on accessory cells in antigen-induced responses of human lymphocytes.
研究了诱导经抗原或丝裂原刺激的人淋巴细胞增殖及特异性抗体反应所需的辅助细胞。一种Ia阴性的人髓样肿瘤细胞系K562，在对商陆丝裂原（PWM）刺激的单核细胞耗竭淋巴细胞增殖中可替代单核细胞。K562细胞在PWM诱导的来自钥孔戚血蓝蛋白（KLH）免疫供体的细胞抗KLH抗体合成中也可作为辅助细胞。相比之下，仅单核细胞而非K562细胞可在抗原诱导的淋巴细胞增殖以及抗原诱导的、抗原特异性抗体产生中作为辅助细胞。然而，K562细胞与单核细胞一样，能够正负调节多克隆免疫球蛋白反应。因此，表达Ia的辅助细胞可在抗原诱导的增殖和抗体反应中发挥作用，而不表达Ia的细胞可在丝裂原诱导而非抗原诱导的反应中发挥作用。这些研究表明在抗原诱导与丝裂原诱导的人淋巴细胞反应中所需辅助细胞的性质存在二分法，并强烈提示Ia或Ia相关分子在人淋巴细胞抗原诱导反应的辅助细胞上具有必不可少的作用。
