Pette D, Tyler K R
J Physiol. 1983 May;338:1-9. doi: 10.1113/jphysiol.1983.sp014655.
Succinate dehydrogenase activity (SDH) was estimated kinetically in individual muscle fibres from the rabbit tibialis anterior, in cryostat sections using computer-linked microphotometry to record initial reaction velocities. These were correlated with fibre type based on myofibrillar actomyosin ATPase staining. Analysis of type IIA and IIB fibre populations in control muscles demonstrated wide variations in SDH activity between fibres of identical myosin ATPase type, with a considerable overlap in oxidative activities of the IIA and IIB populations. Muscles chronically stimulated via the peroneal nerve, using two different frequency patterns, showed increases in SDH activity which were primarily located in the type IIB fibres. This increase was observed both in muscles stimulated continuously at 10 Hz, and when similar numbers of stimuli were applied in brief trains at higher frequency. An earlier onset and more rapid rate of increase of SDH activity was seen with 10 Hz stimulation than with higher frequency, though the levels after 14 days of either pattern of stimulation were not significantly different.
采用计算机连接的显微光度法记录初始反应速度，在低温恒温器切片中对兔胫前肌的单个肌纤维进行琥珀酸脱氢酶活性（SDH）的动力学估计。这些活性与基于肌原纤维肌动球蛋白ATP酶染色的纤维类型相关。对对照肌肉中IIA型和IIB型纤维群体的分析表明，相同肌球蛋白ATP酶类型的纤维之间SDH活性存在广泛差异，IIA型和IIB型群体的氧化活性有相当大的重叠。通过腓总神经使用两种不同频率模式进行慢性刺激的肌肉，其SDH活性增加，主要位于IIB型纤维中。在以10Hz持续刺激的肌肉中以及以更高频率在短串中施加相似数量刺激时，均观察到这种增加。与更高频率刺激相比，10Hz刺激时SDH活性出现更早且增加速度更快，不过两种刺激模式14天后的水平无显著差异。
