Pattus F, Desnuelle P, Verger R
Biochim Biophys Acta. 1978 Feb 2;507(1):62-70. doi: 10.1016/0005-2736(78)90374-7.
Two types of film structure are formed when liposomes are spread at the air/water interface. At zero surface pressure, there is a slow transformation of the closed bilayered structure into a lipid monolayer. The internal content of the liposomes is released into the aqueous subphase. In contrast, when multilamellar liposomes are spread against a surface pressure, they retain their internal content at the air/water interface by forming multilayered structures. Among the liposomes which dipped through the interface an important fraction loses its internal content. During the spreading process at zero surface pressure, it seems that the outer layer of the liposome spreads with a better yield as compared with the inner layer. It is possible to use this spreading technique to determine the asymmetrical distribution of lipids across bilayers.
当脂质体在空气/水界面铺展时会形成两种类型的膜结构。在零表面压力下，封闭的双层结构会缓慢转变为脂质单层。脂质体的内部物质会释放到水相下层中。相比之下，当多层脂质体抵抗表面压力铺展时，它们通过形成多层结构将内部物质保留在空气/水界面。在浸入界面的脂质体中，有相当一部分会失去其内部物质。在零表面压力下的铺展过程中，脂质体的外层似乎比内层铺展得更好。可以利用这种铺展技术来确定脂质在双层膜中的不对称分布。
