Dearfield K L, Jacobson-Kram D, Williams J R
Cytogenet Cell Genet. 1984;38(3):216-20. doi: 10.1159/000132063.
We have investigated spontaneous sister chromatid exchange (SCE) frequencies in peripheral lymphocytes and bone marrow cells explanted from two strains of the laboratory rat, Fischer 344 and Sprague-Dawley. A small, but significant difference was noted for both cell types, with the Fischer 344 rat being consistently higher. Other cell parameters, such as the mitotic index and the replicative index, were similar in the two strains. SCE levels in cultured peripheral lymphocytes after intraperitoneal administration of the alkylating drug cyclophosphamide (10 mg/kg) were similar for the two strains. Fischer 344 rats are known to have approximately a 10-fold higher incidence of spontaneous leukemia than do Sprague-Dawley rats. Since SCE frequency is a sensitive measure of DNA damage, our observations suggest that high leukemia incidence in the Fischer 344 rat may be related to a higher level of spontaneous DNA damage.
我们研究了从两种实验大鼠品系（Fischer 344和Sprague-Dawley）分离出的外周淋巴细胞和骨髓细胞中的自发姐妹染色单体交换（SCE）频率。两种细胞类型均观察到一个小但显著的差异，Fischer 344大鼠的SCE频率始终较高。其他细胞参数，如有丝分裂指数和复制指数，在这两个品系中相似。腹腔注射烷化剂环磷酰胺（10 mg/kg）后，两种品系培养的外周淋巴细胞中的SCE水平相似。已知Fischer 344大鼠自发白血病的发生率比Sprague-Dawley大鼠高约10倍。由于SCE频率是DNA损伤的敏感指标，我们的观察结果表明，Fischer 344大鼠白血病高发可能与较高水平的自发DNA损伤有关。
