D-赤藓糖对鱼腥藻7120全藻丝中固氮酶活性的影响。
Effects of D-erythrose on nitrogenase activity in whole filaments of Anabaena sp. strain 7120.
作者信息
Privalle L S
出版信息
J Bacteriol. 1984 Nov;160(2):794-6. doi: 10.1128/jb.160.2.794-796.1984.
Abstract
D-Erythrose, which has been shown to enhance nitrogenase activity (acetylene reduction) by isolated heterocysts, was studied for its effects on nitrogenase activity and nitrite uptake by whole filaments of Anabaena sp. strain 7120. D-Erythrose had little effect on acetylene reduction in the light; however, at a concentration of 10 mM, it could restore 3'-(3,4-dichlorophenyl)-1',1'-dimethyl urea-inhibited or dark-limited levels to light-supported levels. Sucrose, glucose, or fructose did not exhibit similar effects. D-Erythrose had little effect on nitrite uptake, an indirect measure of nitrite reductase activity by nitrate-grown whole filaments. It was concluded that erythrose effects were mediated by heterocysts and were therefore specific for nitrogenase.
摘要
已证明D-赤藓糖可增强分离的异形胞的固氮酶活性（乙炔还原），本研究考察了其对鱼腥藻7120全藻丝固氮酶活性和亚硝酸盐摄取的影响。D-赤藓糖对光照下的乙炔还原作用很小；然而，在浓度为10 mM时，它可将3'-(3,4-二氯苯基)-1',1'-二甲基脲抑制的或黑暗限制的水平恢复到光照支持的水平。蔗糖、葡萄糖或果糖未表现出类似作用。D-赤藓糖对亚硝酸盐摄取影响很小，亚硝酸盐摄取是硝酸盐培养的全藻丝中亚硝酸盐还原酶活性的间接指标。得出的结论是，赤藓糖的作用是由异形胞介导的，因此对固氮酶具有特异性。
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