Checiu M, Sandor S, Gârban Z
Morphol Embryol (Bucur). 1984 Jul-Sep;30(3):175-84.
In completion of the previously outlined "experimental alcohol blastopathy", the role of acetaldehyde in the induction of preimplantation pathological changes in rat embryos has been controlled. Two experimental models were used: the direct administration of acetaldehyde by gavage and the blockage of acetaldehyde metabolization by ANTALCOL (an aldehyde-dehydrogenase blocking compound). The main results were as follows: The exogenous acetaldehyde in the blood of pregnant animals has an obvious effect upon the developmental rate during the late preimplantation period (retarding segmentation, blastulation), and in one of the experimental models upon the oviductal-uterine migration rate. The increase of the blood acetaldehyde level by blockage of its further metabolization has a more marked effect as compared with the direct intravenous administration of the substance. According to our previous observations the intravenous application of ethanol on the same day (day 4) has no such effect. The direct noxious influence upon the developing preimplantation embryos (fragmentation) of the increased level of acetaldehyde obtained by ANTALCOL treatment is similar but more marked than this effect obtained previously by ethanol administration. The same effect observed after the direct administration of the substance is less marked than the effect of ANTALCOL treatment but more marked than the effect of intravenous ethanol administration. These results attest that acetaldehyde may contribute (alone or together with the effect of ethanol) to the induction of "experimental alcohol blastopathy". The less marked action of the substance proper introduced into the blood stream may be due--in our opinion--to its possible alteration during the period between distillation and application.
在完成先前概述的“实验性酒精胚胎病”研究中，已对乙醛在诱导大鼠胚胎植入前病理变化中的作用进行了研究。使用了两种实验模型：通过灌胃直接给予乙醛以及用ANTALCOL（一种醛脱氢酶阻断化合物）阻断乙醛代谢。主要结果如下：妊娠动物血液中的外源性乙醛对植入前后期的发育速度有明显影响（延缓卵裂、囊胚形成），并且在其中一个实验模型中对输卵管 - 子宫迁移速度有影响。与直接静脉注射该物质相比，通过阻断其进一步代谢来提高血液中乙醛水平的影响更为显著。根据我们先前的观察，在同一天（第4天）静脉注射乙醇没有这种效果。ANTALCOL处理导致的乙醛水平升高对发育中的植入前胚胎（碎片化）的直接有害影响与先前乙醇给药所获得的影响相似，但更为显著。直接给予该物质后观察到的相同效果比ANTALCOL处理的效果要小，但比静脉注射乙醇的效果更显著。这些结果证明乙醛可能（单独或与乙醇的作用一起）导致“实验性酒精胚胎病”的发生。我们认为，直接注入血流中的该物质本身作用较小，可能是由于其在蒸馏和应用之间的时间段内可能发生了改变。
