Altenburg B C, Graham D Y, Estes M K
J Gen Virol. 1980 Jan;46(1):75-85. doi: 10.1099/0022-1317-46-1-75.
A systematic ultrastructural analysis of the replication cycle of the simian rotavirus SA11 in permissive MA104 cells was performed under reproducible conditions. At 8 h p.i., small areas of viroplasm were seen adjacent to swollen vesicles of the rough endoplasmic reticulum (rer) containing a few 80 to 90 nm virus particles. At later times, the size and number of these inclusions increased and the rer contained large numbers of the 80 to 90 nm particles as well as 52 to 65 nm particles. Infected cells eventually lysed, releasing progeny virus. Other cytological alterations included virus particles sequestered in lysosome-like bodies, 15 to 20 nm tubular structures in the nucleus and/or cytoplasm, convoluted membranes within the rer, filament bundles associated with virus particles, and mitochondria containing 1 to 5 virus particles. In addition, SA11 replication was studied in several less permissive cell lines. The results were similar to those with MA104 cells except that a smaller percentage of the cells were productively infected.
在可重复的条件下，对猿猴轮状病毒SA11在允许性MA104细胞中的复制周期进行了系统的超微结构分析。感染后8小时，在含有一些80至90纳米病毒颗粒的粗面内质网（rer）肿胀小泡附近可见小面积的病毒质。在随后的时间里，这些内含物的大小和数量增加，粗面内质网中含有大量80至90纳米的颗粒以及52至65纳米的颗粒。受感染的细胞最终裂解，释放出子代病毒。其他细胞学改变包括病毒颗粒被隔离在溶酶体样小体中、细胞核和/或细胞质中有15至20纳米的管状结构、粗面内质网内的卷曲膜、与病毒颗粒相关的细丝束以及含有1至5个病毒颗粒的线粒体。此外，还在几种较不允许的细胞系中研究了SA11的复制。结果与MA104细胞相似，只是被有效感染的细胞比例较小。
