Suzuki H, Kutsuzawa T, Konno T, Ebina T, Ishida N
Arch Virol. 1981;70(1):33-41. doi: 10.1007/BF01320791.
Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells was observed. The virus antigen in the cytoplasm was detected by indirect immunofluorescence twelve hours after infection. The cytopathic effect occurred 24 hours after infection when virus particles were detected by EM in the culture fluid as well as in thin sections of the infected cells. Virus particles were observed in the dilated RER, nuclear envelope (perinuclear space), viroplasm, and a lysosome-like body. Three types of virus particles were noted: double-shelled particles 75-85 nm in diameter, single-shelled particles 64-68 nm in diameter and electron-dense nucleoids or cores 32-40 nm in diameter. The outer shell of virus particles was acquired by budding through the membrane of the dilated RER. Tubular structures, similar in diameter to the single-shelled particles, were found in the cytoplasm and nucleus of infected MA 104 cells. Bundles of filaments or the leaflet-like inclusion bodies of membrane-bounded bundles of filaments were found in the cytoplasm and seemed to be associated with virus particles.
观察了人轮状病毒2型Wa株在MA 104细胞中的形态发生。感染后12小时，通过间接免疫荧光检测到细胞质中的病毒抗原。感染后24小时出现细胞病变效应，此时在培养液以及感染细胞的超薄切片中通过电子显微镜检测到病毒颗粒。在扩张的粗面内质网、核膜（核周间隙）、病毒质和类似溶酶体的小体中观察到病毒颗粒。注意到三种类型的病毒颗粒：直径75 - 85nm的双层颗粒、直径64 - 68nm的单层颗粒以及直径32 - 40nm的电子致密核仁或核心。病毒颗粒的外壳通过从扩张的粗面内质网的膜出芽获得。在感染的MA 104细胞的细胞质和细胞核中发现了直径与单层颗粒相似的管状结构。在细胞质中发现了成束的细丝或膜结合细丝束状的小叶状包涵体，似乎与病毒颗粒有关。
