Saif L J, Theil K W, Bohl E H
J Gen Virol. 1978 May;39(2):205-17. doi: 10.1099/0022-1317-39-2-205.
The morphogenesis of porcine rotavirus was similar in vitro in porcine kidney (PK) cell cultures and in vivo in porcine epithelial cells as examined by electron microscopy. Infected cells contained cytoplasmic, non-membrane-bound viroplasm and accumulations of virus particles within cisternae of the rough endoplasmic reticulum (RER). Three types of virus particles were noted: double-shelled or complete particles which averaged 77 nm in diam.; single-shelled or naked particles which ranged from 50 to 55 nm in diam.; and electron-dense nucleoids, or cores, 31 to 38 nm in diam. Virus particles acquired outer shells by budding through either matrices of granular, electron-dense viroplasm or membranes of distended RER. Accumulation of numerous single-shelled particles was observed only in PK cell cultures containing a high percentage of infected cells. In these cells, virus release occurred through disruption of the plasma membrane. Tubules, similar in diameter to the single-shelled particles, were observed in the nuclei of a few infected PK cells.
通过电子显微镜检查发现，猪轮状病毒的形态发生在猪肾（PK）细胞培养物的体外环境和猪上皮细胞的体内环境中相似。受感染的细胞含有细胞质中无膜结合的病毒质以及粗面内质网（RER）池内的病毒颗粒聚集体。观察到三种类型的病毒颗粒：直径平均为77nm的双层或完整颗粒；直径在50至55nm之间的单层或裸露颗粒；以及直径为31至38nm的电子致密核仁或核心。病毒颗粒通过在颗粒状、电子致密病毒质的基质或扩张的RER膜上出芽获得外壳。仅在含有高比例受感染细胞的PK细胞培养物中观察到大量单层颗粒的积累。在这些细胞中，病毒通过质膜破裂释放。在少数受感染的PK细胞核中观察到直径与单层颗粒相似的小管。
