猿猴病毒40转化的细胞系中病毒基因组的转录。I. 病毒特异性核RNA的图谱绘制。
Transcription of the viral genome in cell lines transformed by simian virus 40. I. Mapping of virus-specific nuclear RNAs.
作者信息
Chumakov P M
出版信息
Nucleic Acids Res. 1980 Jan 11;8(1):111-25. doi: 10.1093/nar/8.1.111.
Abstract
Mapping of virus-specific nuclear transcripts was carried out in three lines of rat cells transformed by SV40. Each of these cell lines contained a single copy of integrated viral DNA with identified regions adjacent to cell DNA (1). The main virus-specific nuclear transcript in all of these cell lines was shown to be complementary to the minus strand of the early region in SV40 genome. Each cell nucleus contained approximately 50 copies of these RNAs. Transcripts complementary to both strands of the late region in viral genome were also detectable in all of these cell lines. Its content varied depending on the cell line and was 20-50-fold less than that of the main virus-specific transcript. All the regions of integrated SV40 genome in isolated nuclei of transformed cells were equally sensitive to pancreatic DNase I treatment suggesting that the whole viral genome served as a template for RNA synthesis in these cell lines.
摘要
在由SV40转化的三株大鼠细胞系中进行了病毒特异性核转录本的定位。这些细胞系中的每一个都含有整合病毒DNA的单拷贝，其与细胞DNA相邻的区域已确定(1)。所有这些细胞系中的主要病毒特异性核转录本显示与SV40基因组早期区域的负链互补。每个细胞核含有大约50个拷贝的这些RNA。在所有这些细胞系中也可检测到与病毒基因组晚期区域两条链互补的转录本。其含量因细胞系而异，比主要病毒特异性转录本的含量少20 - 50倍。转化细胞分离核中整合的SV40基因组的所有区域对胰DNA酶I处理同样敏感，这表明整个病毒基因组在这些细胞系中作为RNA合成的模板。
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