Khoury G, Byrne J C, Martin M A
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1925-8. doi: 10.1073/pnas.69.7.1925.
Small amounts of fractionated, denatured, (32)P-labeled DNA from SV40 virus were incubated with a large excess of the complementary RNA of SV40 prepared in vitro with Escherichia coli RNA polymerase; the viral DNA strands were separated on hydroxyapatite columns. The RNA present in green monkey cells late in the lytic cycle reacted with 40-42% of the strand complementary to the in vitro complementary RNA (minus strand), and 60-64% of the opposite (plus) strand. "Early lytic" RNA failed to significantly interact with the plus strand, but formed stable duplex molecules with 35-39% of the minus strand. The RNA prepared from mouse embryo cells 24 hr after infection with SV40 combined with 35-38% of the minus strand and 60-62% of the plus strand. In all cases, the same regions of either the plus or minus strand appear to be transcribed in permissive and nonpermissive infections.
将少量经分级分离、变性的、用³²P标记的猴空泡病毒40（SV40）病毒DNA，与用大肠杆菌RNA聚合酶体外制备的大量过量的SV40互补RNA一起温育；病毒DNA链在羟基磷灰石柱上分离。在裂解周期后期的绿猴细胞中存在的RNA，与体外互补RNA（负链）互补的链的40 - 42%发生反应，与相反（正）链的60 - 64%发生反应。“早期裂解”RNA未能与正链发生显著相互作用，但与35 - 39%的负链形成稳定的双链分子。感染SV40 24小时后从小鼠胚胎细胞制备的RNA，与35 - 38%的负链和60 - 62%的正链结合。在所有情况下，正链或负链的相同区域似乎在允许和非允许感染中都被转录。
