Oskarsson M, McClements W L, Blair D G, Maizel J V, Vande Woude G F
Science. 1980 Mar 14;207(4436):1222-4. doi: 10.1126/science.6243788.
A 15.0-kilobase (kb) Eco RI DNA fragment from normal mouse Balb/c genomic DNA that contains sequences (sarc) homologous to the acquired cell sequences (src) of Moloney sarcoma virus (MSV) has been cloned in phage lambda. The sarc region (1.2 to 1.3 kb) of the 15.0-kb cell fragment is indistinguishable from the src region of two isolates of MSV as judged by heteroduplex and restriction endonuclease analyses. The cellular sequences flanking sarc show no homology to other MSV sequences. Whereas cloned subgenomic portions of MSV that contain src transformed NIH-3T3 cells in vitro, the cloned sarc fragment is inactive.
从正常小鼠Balb/c基因组DNA中分离得到一个15.0千碱基对（kb）的Eco RI DNA片段，该片段含有与莫洛尼肉瘤病毒（MSV）的获得性细胞序列（src）同源的序列（sarc），已被克隆到噬菌体λ中。通过异源双链分析和限制性内切酶分析判断，15.0-kb细胞片段的sarc区域（1.2至1.3 kb）与MSV两个分离株的src区域无法区分。sarc侧翼的细胞序列与其他MSV序列无同源性。虽然含有src的MSV克隆亚基因组部分在体外可转化NIH-3T3细胞，但克隆的sarc片段无活性。
