莫洛尼鼠肉瘤病毒的分子克隆：正常小鼠基因组内病毒相关序列的排列
Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome.
作者信息
Tronick S R, Robbins K C, Canaani E, Devare S G, Andersen P R, Aaronson S A
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6314-8. doi: 10.1073/pnas.76.12.6314.
The unintegrated circular DNA form of Moloney murine sarcoma virus (MSV) has been cloned in bacteriophage lambda. Discrete deletions in the viral genome were shown to occur during propagation of recombinant phage in Escherichia coli. Heteroduplex and restriction enzyme analyses indicated the deletion of tandemly repeated sequences within certain of the cloned MSV DNA inserts. Cloned MSV DNA was used to prepare a probe composed of its acquired cellular (src) sequences, shown previously to be necessary for MSV transformation. Analysis of EcoRI digests of normal mouse cellular DNA revealed the presence of a single 14-kilobase-pair fragment containing these sequences which lacked contiguity with endogenous type C helper viral information of the same cells. Thus, the sarcoma virus-specific sequences of MSV are represented within the normal mouse genome in a manner analogous to that of a cellular gene.
莫洛尼氏鼠肉瘤病毒（MSV）的未整合环状DNA形式已被克隆到噬菌体λ中。在重组噬菌体于大肠杆菌中繁殖期间，病毒基因组中出现了离散的缺失。异源双链体和限制酶分析表明，在某些克隆的MSV DNA插入片段中，串联重复序列发生了缺失。克隆的MSV DNA被用于制备一个由其获得的细胞（src）序列组成的探针，该序列先前已被证明是MSV转化所必需的。对正常小鼠细胞DNA的EcoRI酶切分析显示，存在一个单一的14千碱基对片段，其中包含这些序列，这些序列与同一细胞的内源性C型辅助病毒信息不连续。因此，MSV的肉瘤病毒特异性序列在正常小鼠基因组中的呈现方式类似于细胞基因。
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