在喂食氯贝丁酯的大鼠的灌注心脏中，芥酸的β-氧化增加。

Increased beta-oxidation of erucic acid in perfused hearts from rats fed clofibrate.

Norseth J

Biochim Biophys Acta. 1980 Feb 22;617(2):183-91. doi: 10.1016/0005-2760(80)90161-7.

Abstract

The metabolism of [14-14C]erucate and [U-14C]palmitate has been investigated in perfused heart from rats fed 0.3% clofibrate for 10 days and from control rats. 2. The total uptake of fatty acids in the heart increased in the clofibrate fed group. Clofibrate increased the oxidation of [14-14C]erucic acid by 100% and the oxidation of [U-14C]palmitic acid by 30% compared to controls. 3. The chain-shortening of erucate to C20:1 and C18:1 fatty acids in the perfused heart was stimulated at least two-fold by clofibrate feeding. 4. The activity of the peroxisomal marker enzyme catalase increased 60%, the activity of cytochrome oxidase increased approx. 16% and the content of total coenzyme A increased 30% in heart homogenates from rats fed clofibrate compared to controls. 5. The isolated mitochondrial fraction from clofibrate fed rats showed an increased capacity for oxidation of palmitoylcarnitine and decanoylcarnitine, while the oxidation of erucoylcarnitine showed little change. 6. It is suggested that clofibrate increases the oxidation of [14-14C]erucic acid in the perfused heart by increasing the capacity for chain-shortening of [14-14C]erucate in the peroxisomal beta-oxidation system.

摘要

对喂食0.3%氯贝丁酯10天的大鼠和对照大鼠的灌流心脏中[14-14C]芥酸酯和[U-14C]棕榈酸的代谢进行了研究。2. 氯贝丁酯喂养组心脏中脂肪酸的总摄取量增加。与对照组相比，氯贝丁酯使[14-14C]芥酸的氧化增加了100%，[U-14C]棕榈酸的氧化增加了30%。3. 氯贝丁酯喂养至少使灌流心脏中芥酸酯链缩短为C20:1和C18:1脂肪酸的过程增加了两倍。4. 与对照组相比，喂食氯贝丁酯的大鼠心脏匀浆中过氧化物酶体标记酶过氧化氢酶的活性增加了60%，细胞色素氧化酶的活性约增加了16%，总辅酶A的含量增加了30%。5. 氯贝丁酯喂养大鼠分离出的线粒体部分显示，棕榈酰肉碱和癸酰肉碱的氧化能力增加，而芥酰肉碱的氧化变化不大。6. 有人提出，氯贝丁酯通过增加过氧化物酶体β氧化系统中[14-14C]芥酸酯链缩短的能力，增加灌流心脏中[14-14C]芥酸的氧化。