脾坏死病毒感染性DNA的形成与结构
Formation and structure of infectious DNA of spleen necrosis virus.
作者信息
Fritsch E, Temin H M
出版信息
J Virol. 1977 Jan;21(1):119-30. doi: 10.1128/JVI.21.1.119-130.1977.
Abstract
The kinetics of formation and the structure of infectious DNA of spleen necrosis virus were determined. Nonintegrated infectious viral DNA first appeared 18 to 24 h after infection of dividing cells and persisted for more than 14 days. The nonintegrated infectious viral DNA was in the form of either a double-stranded linear DNA with a molecular weight of 6 X 10(6), detected in both the cytoplasm and nucleus, or a closed circular DNA of the same molecular weight, detected primarily in the nucleus. Integrated infectious viral DNA appeared soon after the nonintegrated infectious viral DNA and was the predominant form of infectious viral DNA late after infection. Integration of the spleen necrosis virus DNA into the chicken cell genome was demonstrated by three independent criteria. Nucleic acid hybridization indicated that the linear infectious viral DNA had a 5- to 10-fold higher specific infectivity than either the closed circular or integrated infectious viral DNA. Infectious viral DNA did not appear in infected stationary cells, indicating some cellular influence on the formation of infectious viral DNA.
摘要
测定了脾坏死病毒感染性DNA的形成动力学及结构。未整合的感染性病毒DNA在分裂细胞感染后18至24小时首次出现，并持续超过14天。未整合的感染性病毒DNA呈两种形式：一种是分子量为6×10⁶的双链线性DNA，在细胞质和细胞核中均能检测到；另一种是分子量相同的闭环DNA，主要在细胞核中检测到。整合的感染性病毒DNA在未整合的感染性病毒DNA出现后不久出现，并且是感染后期感染性病毒DNA的主要形式。通过三个独立标准证明了脾坏死病毒DNA整合到鸡细胞基因组中。核酸杂交表明，线性感染性病毒DNA的比感染性比闭环或整合的感染性病毒DNA高5至10倍。感染性病毒DNA未出现在受感染的静止细胞中，表明细胞对感染性病毒DNA的形成有一定影响。
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