Rephaeli A W, Artenstein I R, Saier M H
J Bacteriol. 1980 Mar;141(3):1474-7. doi: 10.1128/jb.141.3.1474-1477.1980.
Hydrolysis of sugar phosphates by crude and purified preparations of periplasmic hexose phosphatase from Salmonella typhimurium followed Michaelis-Menten kinetics. The enzyme bound glucose 1-phosphate with high affinity (Km = 10 microM) but bound glucose 6-phosphate with low affinity (Km = 2,000 microM). The order of substrate affinities was glucose 1-phosphate greater than mannose 1-phosphate = galactose 1-phosphate greater than fructose 1-phosphate greater than glucose 6-phosphate. These results and others suggest that the physiological function of the enzyme is the periplasmic hydrolysis of hexose 1-phosphates.
鼠伤寒沙门氏菌周质己糖磷酸酶的粗提物和纯化物对糖磷酸酯的水解遵循米氏动力学。该酶对1-磷酸葡萄糖具有高亲和力（Km = 10微摩尔），但对6-磷酸葡萄糖的亲和力较低（Km = 2000微摩尔）。底物亲和力的顺序为1-磷酸葡萄糖＞1-磷酸甘露糖 = 1-磷酸半乳糖＞1-磷酸果糖＞6-磷酸葡萄糖。这些结果及其他结果表明，该酶的生理功能是周质中己糖1-磷酸酯的水解。
