多瘤大肿瘤抗原对于由病毒DNA介导的肿瘤发生并非必需。

Moore J L, Chowdhury K, Martin M A, Israel M A

Proc Natl Acad Sci U S A. 1980 Mar;77(3):1336-40. doi: 10.1073/pnas.77.3.1336.

The arrangement of viral DNA sequences in a hamster cell line derived from a tumor induced by a recombinant plasmid DNA preparation containing the entire polyoma virus genome was examined. In the recombinant plasmid employed, viral DNA sequences specifying the large species of polyoma tumor antigen but not the small and middle tumor antigens were interrupted by the insertion of plasmid DNA at the EcoRI restriction endonuclease site. Blot-hybridization analyses of tumor cell DNA indicated that the "joints" linking viral and plasmid DNAs in the original recombinant plasmid used in animal inoculation had been preserved. Integration into the hamster cell genome had apparently occurred within plasmid DNA sequences. These results indicate that polyoma large tumor antigen is not required for tumorigenesis mediated by viral DNA.

对源自由包含完整多瘤病毒基因组的重组质粒DNA制剂诱导产生的肿瘤的仓鼠细胞系中的病毒DNA序列排列进行了检查。在所使用的重组质粒中，指定多瘤肿瘤抗原大种但不包括小种和中种肿瘤抗原的病毒DNA序列在EcoRI限制性内切酶位点被质粒DNA插入打断。肿瘤细胞DNA的印迹杂交分析表明，在动物接种中使用的原始重组质粒中连接病毒和质粒DNA的“接头”得以保留。整合到仓鼠细胞基因组中显然发生在质粒DNA序列内。这些结果表明，病毒DNA介导的肿瘤发生不需要多瘤大肿瘤抗原。