Murphy M G, Condon S
Arch Microbiol. 1984 May;138(1):44-8. doi: 10.1007/BF00425405.
Two strains of Lactobacillus plantarum accumulated H2O2 when grown aerobically in a complex glucose based medium. The H2O2 accumulation did not occur immediately on exposure of the culture to O2 but was delayed for a time which, in the case of one strain, was dependent on the amount of inoculum used to seed the culture. The accumulation was always preceded by an increase in the rate of O2 utilization by the cultures. The latter coincided approximately with an increase in specific activity of NADH oxidase, pyruvate oxidase and NADH peroxidase. H2O2 was not a product of NADH oxidase in vitro but was formed in substantial quantities from O2 during oxidation of pyruvate. The three enzymes were induced by O2 and H2O2; the induction of NADH oxidase responded to lower levels of O2 (but not of H2O2) than the pyruvate oxidase or the NADH peroxidase.
两株植物乳杆菌在以葡萄糖为基础的复杂培养基中需氧培养时会积累过氧化氢。当培养物暴露于氧气中时，过氧化氢的积累并非立即发生，而是会延迟一段时间，对于其中一个菌株而言，延迟时间取决于接种到培养物中的接种量。在过氧化氢积累之前，培养物的氧气利用率总是会增加。后者大约与NADH氧化酶、丙酮酸氧化酶和NADH过氧化物酶的比活性增加同时发生。过氧化氢在体外不是NADH氧化酶的产物，但在丙酮酸氧化过程中由氧气大量生成。这三种酶由氧气和过氧化氢诱导；与丙酮酸氧化酶或NADH过氧化物酶相比，NADH氧化酶的诱导对较低水平的氧气（而非过氧化氢）有反应。
