用于检测猪现场血清中伪狂犬病病毒抗体的酶免疫测定法的评估
Evaluation of an enzyme immunoassay for detection of antibodies to pseudorabies virus in porcine field sera.
作者信息
Afshar A, Wright P F, Dulac G C
机构信息
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
出版信息
Can J Vet Res. 1987 Oct;51(4):539-41.
The performance of an indirect enzyme-linked immunosorbent assay and the standard serum neutralization test for detection of antibodies to pseudorabies virus in porcine field sera were compared, using 304 sera from pseudorabies-free pigs in Canada, and 1082 and 580 swine sera from the USA and England, respectively. The sensitivity and specificity of the ELISA relative to the serum neutralization test were in the order of 97 to 98%, with the higher agreement between the tests when a 1:40 dilution of serum samples was used for the ELISA. The indirect ELISA was considered to be a rapid and convenient procedure, offering many advantages over the serum neutralization test for routine serodiagnostic use.
比较了间接酶联免疫吸附试验与标准血清中和试验检测猪现场血清中伪狂犬病病毒抗体的性能，使用了来自加拿大无伪狂犬病猪的304份血清，以及分别来自美国和英国的1082份和580份猪血清。相对于血清中和试验，ELISA的敏感性和特异性约为97%至98%，当血清样本以1:40稀释用于ELISA时，两种试验之间的一致性更高。间接ELISA被认为是一种快速便捷的方法，在常规血清学诊断应用中比血清中和试验具有许多优势。
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