Hübschle O J
Arch Virol. 1980;64(2):133-40. doi: 10.1007/BF01318017.
Bluetongue-virus (BTV) was found to agglutinate a variety of erythrocytes including sheep-, chicken-, guinea pig- and mouse-erythrocytes. Hemagglutination was inhibited specifically with type specific serum. A temperature dependence was only found for chicken erythrocytes, which showed a hemagglutination optimum at 37 degrees C. The hemagglutination was lost upon treatment of the virus with 0.4% trypsin as well as after treatment with 0.01 M KJO4. Heating of the virus preparation to 56 degrees C resulted in the loss of the HA-activity. Gelchromatographic studies indicated that the hemagglutinating capacity is associated with the complete virion. Whereas virulent strains of BTV hemagglutinate a number of different erythrocytes the avirulent type tested produced only a slight hemagglutination with sheep red blood cells. However, specific antiserum produced with the avirulent strain yielded strong hemagglutination inhibition (HI) with the corresponding virulent strain. Treatment of sera prior to their use in the HI proved necessary to remove nonspecific inhibitors. The efficiency of KJO4 in removing nonspecific inhibitors. The efficiency of KJO4 in removing nonspecific inhibitors indicates that carbohydrates represent the major group of nonspecific inhibitors. The data represented recommended the hemagglutination inhibition tests as a new method to identify the various BTV serotypes.
蓝舌病毒（BTV）能凝集多种红细胞，包括绵羊、鸡、豚鼠和小鼠的红细胞。血凝反应能被型特异性血清特异性抑制。仅发现鸡红细胞存在温度依赖性，其在37℃时血凝反应最佳。用0.4%胰蛋白酶处理病毒以及用0.01M高碘酸钾处理后，血凝反应消失。将病毒制剂加热至56℃会导致血凝活性丧失。凝胶色谱研究表明，血凝能力与完整病毒体相关。强毒株BTV能凝集多种不同红细胞，而所检测的无毒株仅与绵羊红细胞产生轻微血凝反应。然而，用无毒株产生的特异性抗血清对相应强毒株产生强烈的血凝抑制（HI）作用。在HI试验中使用血清前进行处理以去除非特异性抑制剂是必要的。高碘酸钾去除非特异性抑制剂的效率表明碳水化合物是主要的非特异性抑制剂类别。这些数据表明血凝抑制试验可作为鉴定不同BTV血清型的新方法。
