爱泼斯坦-巴尔病毒（EBV）DNA片段在pBR32和Charon3A中的克隆。

Cloning of Epstein-Barr virus (EBV) DNA fragments in pBR32 and Charon3A.

作者信息

Yano S, Faber H E, Lee Y S, Nonoyama M

出版信息

Gene. 1981 Mar;13(2):203-8. doi: 10.1016/0378-1119(81)90009-3.

Abstract

Restriction fragments of Epstein-Barr virus (EBV; B95-8) DNA were cloned in the Tc gene of pBR322 (HindIII-F, -G, -I, -J, -K, -L, and -M) and in Charon3A (EcoRI-GI and -G2). Altogether these cloned fragments covered 39% of the entire viral genome. The cloned EcoRI-G2 fragment of EBV (B95-8) DNA was shown to contain, in addition to HindIII-J, two more HindIII-fragments : HindIII-M, which had not been located on the linkage map of the viral genome (Given and Kieff, 1978) and HindIII-N, which had been unrecognized up to now. The utility of this cloning method is discussed in regard to the detailed mapping of a viral genome and large-scale production of the viral DNA.

摘要

爱泼斯坦-巴尔病毒（EBV；B95-8）DNA的限制性片段被克隆到pBR322的Tc基因（HindIII-F、-G、-I、-J、-K、-L和-M）以及Charon3A（EcoRI-GI和-G2）中。这些克隆片段总共覆盖了整个病毒基因组的39%。EBV（B95-8）DNA的克隆EcoRI-G2片段除了含有HindIII-J外，还含有另外两个HindIII片段：尚未定位在病毒基因组连锁图谱上的HindIII-M（Given和Kieff，1978）以及迄今未被识别的HindIII-N。就病毒基因组的详细图谱绘制和病毒DNA的大规模生产而言，讨论了这种克隆方法的实用性。