Raju U, Blaustein A, Levitz M
Steroids. 1980 Jun;35(6):685-95. doi: 10.1016/0039-128x(80)90093-8.
The metabolism of 3H-androstenedione (delta 4-A) and 3H-estriol (E3) was studied in 12 human breast tumors. Part of each tumor was analyzed for estrogen receptor content. Aliquots of tumor homogenates were incubated for 2 hr separately with 3H-delta 4-A and 3H-E3 in the presence of appropriate cofactors. No distinct differences emerged in the profiles of the unconjugated metabolites of 3H-delta 4-A, the major compounds in the approximate order of descendence being androsterone, androstanedione, testosterone, 5 alpha-androstane-3 alpha, 17 beta-diol, epiandrosterone, and dihydrotestosterone. One tumor homogenate from an infiltrating lobular carcinoma converted 3H-delta 4-A to glucosiduronate metabolites (11%), of which androsterone, 6.4%; testosterone, 1.6%; and androstanediol, 0.6% predominated. The homogenate of this tumor and two other tumors converted 3H-E3 to 3H-E3-3S. Conversions of E3 to E3-3S in the other tumor homogenates were less than 0.6%. No correlation between receptor content and the capability of the tumor to conjugate delta 4-A or E3 evolved. However, correlations between steroid hormone metabolism and tumor histopathology may exist.
在12例人类乳腺肿瘤中研究了3H-雄烯二酮（δ4-A）和3H-雌三醇（E3）的代谢。对每个肿瘤的一部分进行雌激素受体含量分析。将肿瘤匀浆的等分试样在适当的辅因子存在下分别与3H-δ4-A和3H-E3孵育2小时。3H-δ4-A的非共轭代谢产物谱未出现明显差异，主要化合物按降序排列大致为雄酮、雄烷二酮、睾酮、5α-雄烷-3α,17β-二醇、表雄酮和二氢睾酮。1例浸润性小叶癌的肿瘤匀浆将3H-δ4-A转化为葡萄糖醛酸代谢产物（11%），其中雄酮占6.4%；睾酮占1.6%；雄烷二醇占0.6%为主。该肿瘤以及其他两个肿瘤的匀浆将3H-E3转化为3H-E3-3S。其他肿瘤匀浆中E3向E3-3S的转化率低于0.6%。受体含量与肿瘤结合δ4-A或E3的能力之间未发现相关性。然而，类固醇激素代谢与肿瘤组织病理学之间可能存在相关性。
