大肠杆菌K2荚膜抗原的结构，一种类磷壁酸聚合物。
Structure of the Escherichia coli K2 capsular antigen, a teichoic acid-like polymer.
作者信息
Jann K, Jann B, Schmidt M A, Vann W F
出版信息
J Bacteriol. 1980 Sep;143(3):1108-15. doi: 10.1128/jb.143.3.1108-1115.1980.
Abstract
The primary structure of the K2 antigen of Escherichia coli was elucidated by composition, alkaline fragmentation, dephosphorylation with hydrofluoric acid, periodate oxidation, and methylation analysis. The polymer contains galactose in the pyranosidic and furanosidic ring form. It consists of phosphogaolactopyranosyl glycerol and phosphagalactofuranosyl glycerol units in a molar ratio of 2:1. The sequence of these units is not known. The structure of the K2 antigen is reminiscent of that of certain teichoic acids of gram-positive bacteria. Using microprecipitation, it was shown that in the polymer galactoside is immunodominant.
摘要
通过组成分析、碱性裂解、氢氟酸去磷酸化、高碘酸盐氧化和甲基化分析，阐明了大肠杆菌K2抗原的一级结构。该聚合物含有吡喃糖环和呋喃糖环形式的半乳糖。它由磷酸吡喃半乳糖基甘油和磷酸呋喃半乳糖基甘油单元组成，摩尔比为2:1。这些单元的序列尚不清楚。K2抗原的结构让人联想到革兰氏阳性菌的某些磷壁酸。通过微量沉淀法表明，在该聚合物中半乳糖苷具有免疫显性。
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