Nakao K, Oki S, Tanaka I, Nakai Y, Imura H
J Clin Endocrinol Metab. 1980 Nov;51(5):1205-7. doi: 10.1210/jcem-51-5-1205.
Using a specific radioimmunoassay for gamma-melanotropin (gamma-MSH), one of the predicted fragments in the amino-terminal portion of the adrenocorticotropin(ACTH)-beta-lipotropin (beta-LPH) precursor, gamma-MSH-like immunoreactivity (gamma-MSH-LI) was detected in human plasma from 4 of 5 patients with Addison's disease and 2 of 3 patients with Nelson's syndrome. None of the normal subjects or patients with Cushing's disease showed detectable concentrations (more than 150 pg/ml) of gamma-MSH-LI. gamma-MSH-LI was secreted concomitantly with ACTH-like immunoreactivity (ACTH-LI) and beta-endorphin-like immunoreactivity (beta-endorphin-LI) in response to insulin-induced hypoglycemia and the administration of lysine-vasopressin. Conversely, intravenous injection of cortisol lowered plasma concentrations of gamma-MSH-LI concomitantly with those of ACTH-LI and beta-endorphin-LI. Gel chromatographic studies of the plasma extracts showed a single peak of gamma-MSH-LI near the elution position of human beta-LPH. These results suggest that gamma-MSH-LI in human plasma is present as a big form and that this big gamma-MSH is secreted concomitantly with ACTH and beta-endorphin.
利用针对γ-促黑素（γ-MSH）的特异性放射免疫分析法，γ-MSH是促肾上腺皮质激素（ACTH）-β-促脂素（β-LPH）前体氨基末端部分的预测片段之一，在5例艾迪生病患者中的4例以及3例纳尔逊综合征患者中的2例的人血浆中检测到了γ-MSH样免疫反应性（γ-MSH-LI）。正常受试者或库欣病患者均未显示出可检测到的γ-MSH-LI浓度（超过150 pg/ml）。在胰岛素诱导的低血糖以及赖氨酸加压素给药后，γ-MSH-LI与ACTH样免疫反应性（ACTH-LI）和β-内啡肽样免疫反应性（β-内啡肽-LI）同时分泌。相反，静脉注射皮质醇会使γ-MSH-LI的血浆浓度与ACTH-LI和β-内啡肽-LI的血浆浓度同时降低。血浆提取物的凝胶色谱研究显示，在人β-LPH的洗脱位置附近有一个γ-MSH-LI单峰。这些结果表明，人血浆中的γ-MSH-LI以一种大分子形式存在，并且这种大分子γ-MSH与ACTH和β-内啡肽同时分泌。
