Smith J A, Ngui-Yen J H
J Clin Microbiol. 1980 Jul;12(1):18-21. doi: 10.1128/jcm.12.1.18-21.1980.
An alpha-toxin disk test is described in which group B streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of Clostridium perfringens. The test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. A total of 95% of strains of pure group B streptococci tested produced positive reactions within 5 h, and all were positive after overnight incubation, with patterns of synergistic hemolysis readily distinguishable from those seen with group A streptococci. The preparation of disks is well within the scope of most clinical laboratories.
描述了一种α毒素纸片试验，其中B组链球菌完成了由产气荚膜梭菌α毒素部分裂解的绵羊红细胞的溶血过程。该试验在绵羊血琼脂初代分离平板以及纯培养物上均能令人满意地进行。总共95%的受试纯B组链球菌菌株在5小时内产生阳性反应，过夜培养后全部呈阳性，其协同溶血模式易于与A组链球菌的模式区分开来。纸片的制备完全在大多数临床实验室的能力范围内。
