Yilma T, Breese S S
J Gen Virol. 1980 Jul;49(1):225-30. doi: 10.1099/0022-1317-49-1-225.
Fluorescent antibody (FA) studies of cells infected with bovine enterovirus showed cytoplasmic blebs with specific fluorescence to the virus. These structures were also found extracellularly in the debris of lysed cells and were RNA-positive by acridine orange (AO) staining. The morphology of virus-infected cells was further studied by scanning electron microscopy (SEM). Transmission electron microscopy (TEM) with immunoferritin tagging showed the development of long sacs with bilaminated and multilaminated membranes. These sacs had multiple twists at different intervals along their length forming a chain of vesicles. The development and maturation of the virus were observed in these vesicles. A number of virus-containing vesicles were also present extracellularly in the debris of lysed cells. In addition, virus was observed in layers of membranous cisternae closely associated with vacuoles and plasma membrane. Some of the cisternae opened to the extracellular space and appeared to allow the release of the virus. Virus particles were also found in patches and in crystals within the cytoplasmic matrix. Many lysed cells contained fibrils often associated with patches of ferritin-tagged virus. This study presents morphological evidence for the release of the virus in vesicles after cell lysis, via cisternae with openings to the extracellular space, and in cytoplasmic blebs.
对感染牛肠道病毒的细胞进行荧光抗体（FA）研究显示，细胞质泡对该病毒呈现特异性荧光。这些结构在裂解细胞的碎片中也存在于细胞外，并且经吖啶橙（AO）染色呈RNA阳性。通过扫描电子显微镜（SEM）进一步研究了病毒感染细胞的形态。免疫铁蛋白标记的透射电子显微镜（TEM）显示出具有双层和多层膜的长囊泡的形成。这些囊泡在其长度上以不同间隔有多个扭曲，形成一串小泡。在这些小泡中观察到了病毒的发育和成熟。在裂解细胞的碎片中，许多含病毒的小泡也存在于细胞外。此外，在与液泡和质膜紧密相关的膜性池层中观察到了病毒。一些池通向细胞外空间，似乎允许病毒释放。病毒颗粒也存在于细胞质基质中的斑块和晶体中。许多裂解细胞含有通常与铁蛋白标记病毒斑块相关的纤维。这项研究提供了形态学证据，证明病毒在细胞裂解后通过通向细胞外空间的池以及细胞质泡以小泡形式释放。
