Yilma T, McVicar J W, Breese S S
J Gen Virol. 1978 Oct;41(1):105-14. doi: 10.1099/0022-1317-41-1-105.
The pre-lytic release mechanism of foot-and-mouth disease virus was investigated by immunofluorescence, acridine orange staining, and electron microscopy in infected bovine and porcine kidney coverslip cultures. Cells with cytoplasmic fluorescence and which were positive for single stranded RNA with acridine orange staining were observed at 2 h after infection. Scanning electron microscopy showed cytoplasmic blebs in all cultures examined 2 h after infection. Rounded cells with virus inclusions began to appear 3 h after infection. Rounded cells and cytoplasmic blebs were shown to have single stranded RNA by acridine orange staining. Immunofluorescence and transmission electron microscopy with immunoferritin tagging demonstrated foot-and-mouth disease virus in cytoplasmic blebs. This study presents evidence for a pre-lytic release of foot-and-mouth disease virus through virus-containing cytoplasmic blebs emerging from infected cells.
通过免疫荧光、吖啶橙染色和电子显微镜技术，在感染口蹄疫病毒的牛和猪肾盖玻片培养物中研究了口蹄疫病毒的裂解前释放机制。感染后2小时观察到细胞质有荧光且经吖啶橙染色呈单链RNA阳性的细胞。扫描电子显微镜显示感染后2小时检查的所有培养物中均有细胞质小泡。感染后3小时开始出现带有病毒包涵体的圆形细胞。经吖啶橙染色显示，圆形细胞和细胞质小泡含有单链RNA。免疫荧光和免疫铁蛋白标记的透射电子显微镜显示细胞质小泡中有口蹄疫病毒。本研究为口蹄疫病毒通过受感染细胞产生的含病毒细胞质小泡进行裂解前释放提供了证据。
