Bartlett J G, Chang T W, Gurwith M, Gorbach S L, Onderdonk A B
N Engl J Med. 1978 Mar 9;298(10):531-4. doi: 10.1056/NEJM197803092981003.
A substance producing cytotoxicity in tissue culture was detected in stool specimens from all of four patients with pseudomembranous colitis due to antibiotics and in one of 54 with antibiotic-associated diarrhea. These stools also caused enterocolitis when injected intracecally into hamsters. On each occasion, cytotoxicity in tissue culture and enterocolitis in hamsters were neutralized by pretreatment with gas-gangrene antitoxin. The toxicity in both tissue cultures and hamsters could be reproduced with broth cultures of clostridia strains isolated from four of the five stools. These results suggest that toxin-producing clostridia are responsible for antibiotic-associated pseudomembranous colitis.
在4例因抗生素导致的伪膜性结肠炎患者的粪便标本中，以及54例抗生素相关性腹泻患者中的1例粪便标本中，检测到一种在组织培养中产生细胞毒性的物质。将这些粪便经盲肠内注射到仓鼠体内时，也会引发小肠结肠炎。每次，通过用气性坏疽抗毒素预处理，可中和组织培养中的细胞毒性以及仓鼠体内的小肠结肠炎。从5份粪便中的4份分离出的梭状芽孢杆菌菌株的肉汤培养物，能够在组织培养和仓鼠体内重现毒性。这些结果表明，产毒素的梭状芽孢杆菌是抗生素相关性伪膜性结肠炎的病因。
