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1.2 Md DNA序列的直接重复参与了P1质粒R68的位点特异性重组。

Direct repetition of a 1.2 Md DNA sequence is involved in site-specific recombination by the P1 plasmid R68.

作者信息

Leemans J, Villarroel R, Silva B, Van Montagu M, Schell J

出版信息

Gene. 1980 Sep;10(4):319-28. doi: 10.1016/0378-1119(80)90152-3.

DOI:10.1016/0378-1119(80)90152-3
PMID:6253353
Abstract

R68.45, a mutant R68 plasmid, carries a 1.5 Md DNA insertion near its kanamycin-resistance region. This DNA consists of a 1.2 Md DNA repetition of neighbouring R68-DNA and a 0.3 Md "foreign" DNA fragment that is flanked by this direct DNA repeat. This fragment seems to be involved in the formation of R'68.45 plasmids. Duplication of the 1.2 Md DNA sequence is also involved in site-specific recombination events of RP4. This 1.2 Md DNA fragment has the properties of an IS sequence and is denoted IS8.

摘要

R68.45是一种突变的R68质粒，在其卡那霉素抗性区域附近携带一个1.5兆达尔（Md）的DNA插入片段。该DNA由相邻R68-DNA的1.2兆达尔DNA重复序列和一个0.3兆达尔的“外源”DNA片段组成，该片段两侧为这种直接DNA重复序列。这个片段似乎参与了R'68.45质粒的形成。1.2兆达尔DNA序列的重复也参与了RP4的位点特异性重组事件。这个1.2兆达尔的DNA片段具有插入序列（IS）的特性，被命名为IS8。

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Gene. 1980 Sep;10(4):319-28. doi: 10.1016/0378-1119(80)90152-3.
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