Simmons D T
J Virol. 1980 Nov;36(2):519-25. doi: 10.1128/JVI.36.2.519-525.1980.
Tau antigens (also known as cellular or nonviral tumor antigens) were detected in uninfected and simian virus 40-infected monkey cells after immunoprecipitation with serum from hamsters bearing simian virus 40-induced tumours (anti-T serum). These two proteins (56,000 daltons) were digested to similarly sized peptides with various amounts of Staphylococcus aureus V8 protease. The Tau antigen isolated from infected monkey cells was closely related but was not identical to the corresponding protein from human cells transformed by simian virus 40, as determined by two-dimensional mapping of their methionine-labeled tryptic peptides. Hamster cells transformed by various primate papovaviruses (simian virus 40, BK virus, and JC virus) synthesized indistinguishable Tau antigens, as determined by two-dimensional peptide mapping. When tested by the same procedure, these proteins and the ones made in monkey and human cells were found to be related to the Tau antigens isolated from simian virus 40-transformed mouse and rat cells. Based on these results, an "evolutionary tree" was constructed to show the relationship among the methionine-containing tryptic peptides of all of these proteins.
用携带猴病毒40诱导肿瘤的仓鼠血清（抗T血清）进行免疫沉淀后，在未感染和感染猴病毒40的猴细胞中检测到了Tau抗原（也称为细胞或非病毒肿瘤抗原）。这两种蛋白质（56,000道尔顿）用不同量的金黄色葡萄球菌V8蛋白酶消化成大小相似的肽段。从感染猴细胞中分离出的Tau抗原与经猴病毒40转化的人细胞中的相应蛋白质密切相关，但并不相同，这是通过对其甲硫氨酸标记的胰蛋白酶肽段进行二维图谱分析确定的。通过二维肽段图谱分析确定，由各种灵长类乳头瘤病毒（猴病毒40、BK病毒和JC病毒）转化的仓鼠细胞合成的Tau抗原无法区分。当用相同程序进行测试时，发现这些蛋白质以及在猴和人细胞中产生的蛋白质与从猴病毒40转化的小鼠和大鼠细胞中分离出的Tau抗原相关。基于这些结果，构建了一棵“进化树”以展示所有这些蛋白质含甲硫氨酸的胰蛋白酶肽段之间的关系。
