小鼠免疫球蛋白γ2a和γ2b链基因的比较表明，外显子可在多基因家族的基因之间进行交换。
Comparison of mouse immunoglobulin gamma 2a and gamma 2b chain genes suggests that exons can be exchanged between genes in a multigenic family.
作者信息
Ollo R, Auffray C, Morchamps C, Rougeon F
出版信息
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2442-6. doi: 10.1073/pnas.78.4.2442.
Abstract
A 23-kilobase EcoRI DNA fragment coding for the BALB/c immunoglobulin gamma 2a chain was cloned from mouse embryo DNA in the cosmid pJC74, and a nucleotide sequence of 1904 bases was determined for the entire constant region (CH1, CH2, and CH3), the three intervening sequences (IVS 1, IVS 2, and IVS 3) and the 5' and 3' flanking sequences. When the gamma 2a chain nucleotide sequence was compared with the gamma 2b chain nucleotide sequence, the percent homology of corresponding segments (excluding deletion and insertion) was 82% for the 5' flanking sequence, 87% for CH1, 84% for IVS 1, 96% for the hinge, 95% for IVS 2, 94.6% for CH2, 86% for IVS 3, 74% for CH3, 89% for the 3' untranslated region, and 92% for the 3' flanking region. These findings show that different domains of gamma 2a and gamma 2b have independent rates of evolution and that some of the noncoding segments of the gene are more conserved than are adjacent coding segments. Hypotheses on the possible role of IVS is gene evolution and expression are discussed.
摘要
从鼠胚胎DNA中，在黏粒pJC74中克隆出了一个编码BALB/c免疫球蛋白γ2a链的23千碱基对的EcoRI DNA片段，并测定了整个恒定区（CH1、CH2和CH3）、三个间隔序列（IVS 1、IVS 2和IVS 3）以及5'和3'侧翼序列的1904个碱基的核苷酸序列。当将γ2a链核苷酸序列与γ2b链核苷酸序列进行比较时，相应片段（不包括缺失和插入）的同源百分比在5'侧翼序列中为82%，CH1中为87%，IVS 1中为84%，铰链区为96%，IVS 2中为95%，CH2中为94.6%，IVS 3中为86%，CH3中为74%，3'非翻译区为89%，3'侧翼区为92%。这些发现表明，γ2a和γ2b的不同结构域具有独立的进化速率，并且该基因的一些非编码片段比相邻的编码片段更为保守。文中讨论了关于间隔序列在基因进化和表达中可能作用的假说。
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