大麦叶绿体中针对紫外线照射和脱嘌呤DNA的核酸内切酶。
Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts.
作者信息
Velemínský J, Svachulová J, Satava J
出版信息
Nucleic Acids Res. 1980 Mar 25;8(6):1373-81. doi: 10.1093/nar/8.6.1373.
Abstract
Lysates of barley chloroplasts release more radioactivity into acid soluble form from UV-irradiated and alkylated-depurinated E. coli [3H] DNA than from intact DNA. By means of affinity chromatography on depurinated DNA-cellulose and/or UV irradiated DNA-cellulose and by electrophoresis in polyacrylamide gels, four activities on depurinated DNA were separated. One of these contained activity against heavily UV-irradiated /270 J.m-2/ native DNA. In addition, two other nucleases specific towards UV-DNA were separated. One of them was active on native and heat denatured DNA irradiated with 10 J . m-2 UV, whereas the other was predominantly active on native UV-irradiated DNA.
摘要
大麦叶绿体裂解物从紫外线照射和烷基化脱嘌呤的大肠杆菌[³H]DNA中释放到酸溶性形式的放射性比从完整DNA中释放的更多。通过在脱嘌呤DNA-纤维素和/或紫外线照射的DNA-纤维素上进行亲和层析以及在聚丙烯酰胺凝胶中进行电泳，分离出了对脱嘌呤DNA的四种活性。其中一种含有针对重度紫外线照射/270 J.m⁻²/天然DNA的活性。此外，还分离出了另外两种对紫外线DNA具有特异性的核酸酶。其中一种对用10 J.m⁻²紫外线照射的天然和热变性DNA有活性，而另一种主要对天然紫外线照射的DNA有活性。
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