通过DNA-纤维素色谱法从哺乳动物细胞中分离内切核酸酶。
Isolation of endonuclease from mammalian cells by DNA - cellulose chromatography.
作者信息
Caputo A, Zupi G, Clanciulli A
出版信息
Nucleic Acids Res. 1975 Jun;2(6):905-13. doi: 10.1093/nar/2.6.905.
Abstract
By means of DNA-cellulose chromatography an enzyme with endonucleolytic activity has been isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, eluted at 0.7 M NaCl, effects the velocity sedimentation of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely inactive using native as well as heat-denatured DNA.
摘要
通过DNA-纤维素色谱法，从哺乳动物细胞核酸性蛋白组分中分离出一种具有核酸内切酶活性的酶。主要活性组分在0.7M NaCl浓度下洗脱，它会影响紫外线照射和烷基化处理后的DNA的速度沉降，导致分子量降低。该组分对天然DNA和热变性DNA均完全无活性。
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J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
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Proc Natl Acad Sci U S A. 1962 Sep 15;48(9):1670-5. doi: 10.1073/pnas.48.9.1670.
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