用DNA损伤剂处理豌豆（Pisum sativum L.）原生质体可诱导出一种与大肠杆菌RecA具有免疫相关性的39千道尔顿叶绿体蛋白。
Treatment of pea (Pisum sativum L.) protoplasts with DNA-damaging agents induces a 39-kilodalton chloroplast protein immunologically related to Escherichia coli RecA.
作者信息
Cerutti H, Ibrahim H Z, Jagendorf A T
机构信息
Section of Plant Biology, Cornell University, Ithaca, New York 14853.
出版信息
Plant Physiol. 1993 May;102(1):155-63. doi: 10.1104/pp.102.1.155.
Abstract
Organisms must have efficient mechanisms of DNA repair and recombination to prevent alterations in their genetic information due to DNA damage. There is evidence for DNA repair and recombination in plastids of higher plants, although very little is known at the biochemical level. Many chloroplast proteins are of eubacterial ancestry, suggesting that the same could be true for the components of a DNA repair and recombination system. A 39-kD protein, immunologically related to Escherichia coli RecA, is present in chloroplasts of pea (Pisum sativum L.). Bandshift gel assays suggest that it binds single-stranded DNA. Its steady-state level is increased by several DNA-damaging agents. These results are consistent with it being a plastid homolog of E. coli RecA protein, presumably involved in DNA repair and recombination, and with the existence of an SOS-like response in pea leaf cells. Experiments with protein synthesis inhibitors suggest that the 39-kD chloroplast protein is encoded in the nucleus.
摘要
生物体必须具备高效的DNA修复和重组机制，以防止其遗传信息因DNA损伤而发生改变。有证据表明高等植物的质体中存在DNA修复和重组现象，尽管在生化水平上人们对此了解甚少。许多叶绿体蛋白起源于真细菌，这表明DNA修复和重组系统的组成成分可能也是如此。豌豆（Pisum sativum L.）叶绿体中存在一种与大肠杆菌RecA免疫相关的39-kD蛋白。凝胶迁移实验表明它能结合单链DNA。几种DNA损伤剂可提高其稳态水平。这些结果与它是大肠杆菌RecA蛋白的质体同源物相一致，推测其参与DNA修复和重组，也与豌豆叶细胞中存在类似SOS的反应相一致。用蛋白质合成抑制剂进行的实验表明，39-kD叶绿体蛋白是由核基因编码的。
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Treatment of pea (Pisum sativum L.) protoplasts with DNA-damaging agents induces a 39-kilodalton chloroplast protein immunologically related to Escherichia coli RecA.用DNA损伤剂处理豌豆（Pisum sativum L.）原生质体可诱导出一种与大肠杆菌RecA具有免疫相关性的39千道尔顿叶绿体蛋白。
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