Misko I S, Moss D J, Pope J H
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4247-50. doi: 10.1073/pnas.77.7.4247.
The specificity of cytotoxic T cells generated in Epstein-Barr virus (EBV)-infected lymphocyte cultures was investigated, using a 51Cr release assay. Potent cytotoxic T cells with preferred specificity directed to antigens expressed on autologous lymphoblastoid cell line (LCL) target cells were present in 14-day cultures of lymphocytes from EBV-seropositive donors and not from seronegative donors. Moreover, the cytotoxic patterns obtained with a panel of HLA antigen-related and unrelated LCL target cells, supported by unlabeled target inhibition tests, strongly indicate that T cell cytotoxicity to EBV is restricted to HLA antigens.
使用铬-51释放试验,研究了在爱泼斯坦-巴尔病毒(EBV)感染的淋巴细胞培养物中产生的细胞毒性T细胞的特异性。在来自EBV血清阳性供体而非血清阴性供体的淋巴细胞14天培养物中,存在对自体淋巴母细胞系(LCL)靶细胞上表达的抗原具有优先特异性的强效细胞毒性T细胞。此外,一组HLA抗原相关和不相关的LCL靶细胞获得的细胞毒性模式,在未标记靶细胞抑制试验的支持下,强烈表明T细胞对EBV的细胞毒性受限于HLA抗原。