Roland J, Cazenave P A
Ann Immunol (Paris). 1976 Sep-Oct;127(5):633-55.
An allotypic specificity of rabbit immunoglobulins designated as A90 has been studied. The A90 pattern found on the Fab fragment and gamma chain of IgG, is located on the constant region of the Fd fragment of IgG. Aa+ molecules (which carry a specificity of the a series) and Aa- molecules (which lack the a series specificity) isolated from the same serum carry the A90 pattern. A90 distribution in a given serum has been studied in several IgG fractions separated by DEAE cellulose chromatography; the most negatively charged of equally concentrated IgG fractions has ten times more A90 allotypic concentration than positively charged ones. The gene controlling A90 allotypic concentration than positively charged ones. The gene controlling A90 synthesis is not linked to a genes. The percentage of IgG molecules carrying the A90 pattern decreases with the age of the rabbits. Part of the anti-A90 antibodies combine with human IgG (of different subclasses) as well as with sheep and mouse IgG (among which they do not differentiate). The absence of linkage between A90 and a genes suggests that the A90 pattern has a glucidic part. We discuss this hypothesis, comparing some features of the A90 pattern to those of IgG human allotypic pattern for which a glucidic structure has been proposed.
对一种名为A90的兔免疫球蛋白同种异型特异性进行了研究。在IgG的Fab片段和γ链上发现的A90模式，位于IgG的Fd片段的恒定区。从同一血清中分离出的Aa +分子（携带a系列的特异性）和Aa-分子（缺乏a系列特异性）都带有A90模式。已在通过DEAE纤维素色谱法分离的几个IgG组分中研究了给定血清中A90的分布；在同等浓度的IgG组分中，带负电荷最多的组分的A90同种异型浓度比带正电荷的组分高十倍。控制A90同种异型浓度的基因比带正电荷的基因更重要。控制A90合成的基因与a基因不连锁。携带A90模式的IgG分子的百分比随兔子年龄的增长而降低。部分抗A90抗体与人类IgG（不同亚类）以及绵羊和小鼠IgG结合（它们之间没有区别）。A90与a基因之间不存在连锁关系，这表明A90模式具有一个糖部分。我们讨论了这一假设，将A90模式的一些特征与已提出具有糖结构的人类IgG同种异型模式的特征进行了比较。
