Naessens J, Newson J, Williams D J, Lutje V
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Immunology. 1988 Apr;63(4):569-74.
Epitopes specific for IgM on peripheral blood lymphocytes from 47 cattle were examined with three class-specific monoclonal antibodies, IL-A30, IL-A50 and B5/4. In all 47 animals tested, mAb IL-A30 detected a similar percentage of peripheral blood lymphocytes as a mAb that recognizes all immunoglobulin classes. However, in some animals mAbs IL-A50 and B5/4 detected a lower percentage of B cells compared with IL-A30 or the mAb against total Ig. They both reacted with a proportion of the serum IgM from these animals, while IL-A30 reacted with all serum IgM. Therefore, it is probable that mAb-A30 recognizes an IgM isotypic determinant and mAbs IL-A50 and B5/4 recognize different IgM allotypic determinants. Using mAb IL-A30 it was found that the percentage of peripheral blood IgM+ lymphocytes varied widely between healthy cattle (4-31%) but remained constant, with only minor variations, within individual animals.
用三种类别特异性单克隆抗体IL-A30、IL-A50和B5/4检测了47头牛外周血淋巴细胞上IgM特异性表位。在所有47只受试动物中，单克隆抗体IL-A30检测到的外周血淋巴细胞百分比与识别所有免疫球蛋白类别的单克隆抗体相似。然而，在一些动物中，与IL-A30或抗总Ig的单克隆抗体相比，单克隆抗体IL-A50和B5/4检测到的B细胞百分比更低。它们都与这些动物的一部分血清IgM发生反应，而IL-A30与所有血清IgM发生反应。因此，很可能单克隆抗体A30识别一种IgM同种型决定簇，而单克隆抗体IL-A50和B5/4识别不同的IgM同种异型决定簇。使用单克隆抗体IL-A30发现，健康牛外周血IgM+淋巴细胞的百分比差异很大（4%-31%），但在个体动物中保持恒定，只有微小变化。
