Kopecka H, Declève A, Lieberman M, Fry K, Kaplan H S
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6189-93. doi: 10.1073/pnas.77.10.6189.
A comparative study has been made of the susceptibility of fibroblastic cells to transfection by DNA from C57BL/Ka mouse lines producing either fibrotropic or thymotropic retroviruses. DNA isolated from fibroblasts that release a B-ecotropic, fibrotropic virus, BL/Ka(B), was found to transfect fibroblasts of Fv-1bb genotype with release of virus similar to BL/Ka(B). Fv-1nn fibroblasts were also susceptible but expression was delayed, and xenotypic mink lung cells were refractory. In contrast, DNA prepared from a murine T-cell lymphoma line producing a B-ecotropic, thymotropic virus failed to transfect mouse fibroblasts though it transfected a nonproducer T-cell lymphoma line. The data suggest that the Fv-1 and differentiation-specific restriction mechanisms operate at different molecular levels.
对成纤维细胞被来自产生嗜纤维或嗜胸腺逆转录病毒的C57BL/Ka小鼠品系的DNA转染的敏感性进行了一项比较研究。从释放B-亲嗜性、嗜纤维病毒BL/Ka(B)的成纤维细胞中分离出的DNA，被发现可转染Fv-1bb基因型的成纤维细胞，并释放出与BL/Ka(B)相似的病毒。Fv-1nn成纤维细胞也易感，但表达延迟，而异型貂肺细胞则难治。相反，从产生B-亲嗜性、嗜胸腺病毒的小鼠T细胞淋巴瘤系制备的DNA未能转染小鼠成纤维细胞，尽管它转染了一个非生产性T细胞淋巴瘤系。数据表明，Fv-1和分化特异性限制机制在不同的分子水平上起作用。
