猿猴腺病毒7在经爱泼斯坦-巴尔病毒转化的猿猴狨猴淋巴母细胞样细胞中单次传代后的特性。
Properties of simian adenovirus 7 after one single passage in simian marmoset lymphoblastoid cells transformed by Epstein Barr virus.
作者信息
Faucon N, Tournier P, Chardonnet Y
出版信息
Biomedicine. 1980 Oct;32(3):144-7.
Simian adenovirus 7 gave an abortive infection in simian marmoset lymphoblastoid cells, B 95-8 and M 81 (transformed by Epstein Barr Virus) whereas non transformed simian lymphocytes could not replicate this virus. Electron dense incomplete particles with a lower density than standard virus in CsCl gradients were isolated. Virus yields were low and the percentage of cells containing viral antigen as measured by immunofluorescence was 0.01% for B 95-8 cells and still less for the M 81 cells. After a single passage in either lymphoblastic cell lines, they had a reduced oncogenicity in vivo. The polypeptide pattern analysis by PAGE showed some modifications.
猿猴腺病毒7在猿猴狨猴淋巴母细胞系B 95 - 8和M 81（由爱泼斯坦 - 巴尔病毒转化）中引发流产感染，而未转化的猿猴淋巴细胞不能复制这种病毒。分离出了在氯化铯梯度中密度低于标准病毒的电子致密不完全颗粒。病毒产量很低，通过免疫荧光测量，B 95 - 8细胞中含有病毒抗原的细胞百分比为0.01%，M 81细胞中的百分比更低。在任一淋巴母细胞系中传代一次后，它们在体内的致癌性降低。通过聚丙烯酰胺凝胶电泳进行的多肽图谱分析显示出一些变化。
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