影响从枯草芽孢杆菌转化细胞中分离供体来源的高分子量单链脱氧核糖核酸的条件。
Conditions affecting the isolation from transformed cells of Bacillus subtilis of high-molecular-weight single-stranded deoxyribonucleic acid of donor origin.
作者信息
Davidoff-Abelson R, Dubnau D
出版信息
J Bacteriol. 1973 Oct;116(1):146-53. doi: 10.1128/jb.116.1.146-153.1973.
Abstract
The deoxyribonucleic acid (DNA) extraction procedure of Piechowska and Fox was evaluated to determine which steps are required for the isolation of high-molecular-weight single-stranded material from transformed cultures of Bacillus subtilis. The results indicate that high-molecular-weight single-stranded DNA can be isolated when certain basic proteins are present at the time of lysis. In the absence of such protective agents as lysozyme or cytC the single-stranded DNA is degraded. The single-stranded DNA can also be protected by being treated with lysozyme at low temperature. The high molecular weight of this single-stranded material and its kinetics of appearance are consistent with its being an intermediate in the transformation process.
摘要
对皮乔夫斯卡和福克斯的脱氧核糖核酸（DNA）提取程序进行了评估，以确定从枯草芽孢杆菌转化培养物中分离高分子量单链物质需要哪些步骤。结果表明，当在裂解时存在某些碱性蛋白质时，可以分离出高分子量单链DNA。在没有溶菌酶或细胞色素C等保护剂的情况下，单链DNA会被降解。单链DNA也可以通过在低温下用溶菌酶处理来保护。这种单链物质的高分子量及其出现的动力学与它作为转化过程中的一种中间体是一致的。
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