Martin R, Cetin Y, Fehm H L, Fahlbusch R, Voigt K H
Am J Pathol. 1982 Mar;106(3):332-41.
Transsphenoidally removed samples of pituitary adenomas from 14 patients with Cushing's disease and 5 patients with Nelson's syndrome always contained groups of uniform small ACTH-cells. Antibodies against the pro-opiocortin precursor fragments beta-endorphin, ACTH, and 16k-peptide recognized material in typical adenoma cells. A subpopulation of these cells, varying in number from sample to sample, specifically exhibited alpha-melanotropin immunoreactivity. Most periadenomatous samples showed signs of severe degeneration. Typical Crooke cells only occurred in samples from patients with Cushing's syndrome, but, with this exception, no clear differences between pituitaries of patients with Cushing's and Nelson's syndromes could be discerned. Two other forms of ACTH-immunoreactive cells were observed: rare, single, highly immunoreactive cells, with characteristics of both normal and Crooke cells, and numerous syncytial groups of cells in an advanced state of disintegration, presumably the remnants of hyperplastic follicles. The four different corticotrophs are characterized according to their fine structure and immunoreactivity in this study.
对14例库欣病患者和5例尼尔森综合征患者经蝶窦切除的垂体腺瘤样本进行检测，结果显示样本中始终存在成群的形态一致的小促肾上腺皮质激素（ACTH）细胞。针对阿片促黑激素皮质素原前体片段β-内啡肽、促肾上腺皮质激素和16k肽的抗体能够识别典型腺瘤细胞中的物质。这些细胞中的一个亚群，数量因样本而异，特异性地表现出α-促黑素细胞激素免疫反应性。大多数腺瘤周围样本显示出严重退变的迹象。典型的克鲁克细胞仅出现在库欣综合征患者的样本中，但除此之外，库欣综合征患者和尼尔森综合征患者的垂体之间没有明显差异。还观察到另外两种形式的ACTH免疫反应性细胞：罕见的单个高免疫反应性细胞，兼具正常细胞和克鲁克细胞的特征；以及大量处于解体晚期的合胞体细胞群，推测为增生性滤泡的残余物。本研究根据四种不同促肾上腺皮质激素细胞的精细结构和免疫反应性对其进行了特征描述。
