使用酶联免疫吸附测定法检测和定量牛血清中针对口蹄疫病毒的IgM、IgA、IgG1和IgG2抗体。
Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay.
作者信息
Abu Elzein E M, Crowther J R
出版信息
J Hyg (Lond). 1981 Feb;86(1):79-85. doi: 10.1017/s0022172400068765.
Abstract
A simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.
摘要
本文描述了一种简单的固相酶免疫测定法，用于检测牛血清中针对口蹄疫（FMD）病毒具有活性的抗体类别。该测定法通过将特异性抗体类别免疫吸附到微量滴定板的类别特异性免疫球蛋白包被孔中，实现了从其他血清蛋白中初步分离特异性抗体类别。特异性抗体与口蹄疫病毒反应，然后通过酶标记的抗病毒IgG进行检测。
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Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay.使用酶联免疫吸附测定法检测和定量牛血清中针对口蹄疫病毒的IgM、IgA、IgG1和IgG2抗体。
J Hyg (Lond). 1981 Feb;86(1):79-85. doi: 10.1017/s0022172400068765.
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本文引用的文献
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PURIFICATION OF RADIOACTIVE FOOT-AND-MOUTH DISEASE VIRUS.放射性口蹄疫病毒的纯化
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Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.用戊二醛将酶与蛋白质偶联。偶联物用于抗原和抗体的检测。
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Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research.酶联免疫吸附测定技术在口蹄疫病毒研究中的应用
J Hyg (Lond). 1978 Jun;80(3):391-9. doi: 10.1017/s0022172400024840.
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Evaluation of enzyme-linked immunosorbent assay for quantitation by subclass for bovine antibodies.牛抗体亚类定量酶联免疫吸附测定法的评估
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